This a squeal to this: https://derpibooru.org/images/3357245

We see Twilight pacing around the corridor, looking really upset and worried while Spike watches on

Twilight: Really upset and guilty What have I done...? What did I do...? What was I thinking...?

Spike: You can't keep blaming yourself, Twilight. Just blame yourself once and move on.

Twilight: Still upset I do blame myself... I blamed these poor little fillies for something that they didn't even do... It's my fault that they've run away...

Just then, the rest of the Mane Six walk in

Twilight: Hopeful Well? Did you find them?

Applejack: Sad Nope... Nothin'...

Rarity: Also sad We can't find them anywhere...

Rainbow: Sad as well They've disappeared...

Hearing this, Twilight feels even worse

Twilight: Even worse Oh, sweet Celestia... Look what I've done... Not allowing those girls to take part in my school... Accusing them for doing thing they didn't do... Those poor fillies... I've been a terrible friend...

Fluttershy: Worried Oh, Twilight...

Pinkie: Also worried You're not a terrible friend...

Twilight: Upset Yes, I am... I screwed things up big time... I was supposed to be the Princess of Friendship... A pony who cares about friends getting along with friends... But I forgot all of that when I blamed the Cutie Mark Crusaders... I was just as bad as Chancellor Neighsay when he falsely accused the other creatures of using friendship as a weapon... Who knows how long until things get worse...

Applejack, Rarity and Rainbow: Worried Twilight...

Twilight: Walks off, now feeling even more upset The girls are right... I am a fraud... I am a fake... I'm no Headmare or Princess of Friendship... Maybe I have let them joined my school... Maybe I shouldn't have thought of this stupid school in the first place... Sniffs as her voice starts to crack Or better yet...maybe it would have been better if I was never born... I'm... I'm just a mistake... Starts crying as she walks off

The rest of the girls and Spike look at each other with sadness

Fluttershy: Sad Oh, dear...

Pinkie: Also sad Poor Twilight...

Rarity: Upset And to think, I thought this school was a good idea..

Applejack: Also upset So did I...

Rainbow: Guilty Perhaps if we had let the girls join our classes and spend more time with them this wouldn't have happened...

Spike: Sighs sadly It wouldn't have helped... If only that Cozy Glow hadn't done what she had did... Failing her test on propose! What a ungrateful little brat! It's no wonder the girls run off...

Applejack: Sad Even so, we're just as bad...

Rarity: Also sad I just hope we can find them soon...

Rainbow: Really sad If anything happens to them, I'll forgive myself...

Headcanon:

Scootaloo is adopted by Bow Hothoof and Windy Whistles.

The events of Season 8-9 (With the exception of The Washouts and Best Gift Ever) and Rainbow Roadtrip are not canon.

Note: This picture is based on these:
https://youtu.be/Y5cMfrFAAP8?si=ocyYvIktkT50CuXB
https://www.fimfiction.net/story/472209/1/forgiveness-denied/no-forgiveness-for-anyone
https://dai.ly/xyogip